![]() (I) Finally, NKĬells lack B cell and T cell markers (CD19–CD3–) and express CD56. (H, K) CD62L identifies naive (TN) CD4 and CD8 T cells, while HLA-DR is expressed by activated T cells (TA). (G) T cells can be further subdivided into CD4+ (T helper cells) and CD8+ (cytotoxic T cells) subpopulations, while (J) regulatory T cells expressĬD4 and CD25. (E) B cells can be further characterized by HLA-DR and CD45RA expression. Get your flow cytometry panels designed in 4 easy steps. With a footprint similar to that of a microwave oven, the Attune NxT can be configured with up to 4 spatially separated lasers (405 nm, 488 nm, 561 nm, and 637 nm) and 16. Saved panels can still be used to communicate with the experts for Free Panel Design Service. The Attune NxT cytometer was therefore designed with more laser and detection channel choices while keeping the same benchtop size of the original 2-laser Attune system. ![]() Their expression of CD3 (T cells), CD19 (B cells), or neither (NK cells). First, complete the panel design service request form our experts will then contact you by email/phone as needed. (F) Within the lymphocyte gate, immune cells can be subdivided based on ![]() Lymphocytes on the scatter plot and express both CD14 and CD33. (C) Lymphocytes and monocytes were identified based on forward and side scatter profiles. (B) CD45+ gating was used to select the leukocyte population from the (A) Dead cells were excludedįrom the analysis by gating on live (propidium iodide–) cells in a dot plot. Were stained as described in the application note and acquired and analyzed on the Attune NxT Flow Cytometer. By clicking Accept All Cookies, you agree to the storing of cookies on your device to enhance site navigation, analyze site usage, and assist in our marketing. Gating strategy for 13-color immunophenotyping analysis of stained human whole blood using a stain/lyse protocol. The ThermoFisher Attune NxT Flow Cytometer combines precision with performance in a true benchtop flow cytometer with four lasers (405, 488, 561 and 637 nm) and 16 parameters of detection. A wide range of reagent choices-as well as the system’s automated compensation module, four spatially separated lasers, and 14 color choices-help to simplify multicolor panel design. Strong signal separation in the Attune NxT Flow Cytometer shows excellent separation of cell populations into subsets for immunophenotyping. Flow cytometry is the method of choice for identifying cells within complex heterogeneous populations, as it allows for multiparameter analysis of thousands to millions of cells in a short time. One classic flow cytometry application is immunophenotyping. Network with experts in the field of flow cytometry at our next user meeting Gain new knowledge for your research, meet new colleagues and get your. See our Sample Data page for examples spanning infectious disease research, fluorescent proteins, CRISPR gene editing, immuno-oncology research, microbiology, plant ploidy, platelets, stem cells, and T cells. With the autosampler attachment and optional passive cooling system, the Attune can run large numbers of low volume samples in plate format.The Attune NxT Flow Cytometer shares common features with the Attune CytPix model.Īlmost any standard flow cytometry application can benefit from the high sensitivity and throughput of the Attune NxT Flow Cytometer. The Attune NxT software is intuitive and easy to learn, and prompts users with step-by-step instructions for cleaning cycles and shutdown procedures. In addition, the Attune NxT has a positive-displacement syringe pump system for sample delivery, allowing volumetric analysis. This enables faster sample throughput rates and minimizes clogs, and allows a broad range of cell and sample types. The Thermo Fisher Attune NxT is a compact, efficient, and flexible benchtop analyzer that utilizes acoustic-assisted hydrodynamic focusing. Attune NxT desktop folder Attune NxT User Guides Attune NxT desktop folder Helpful Attune information includes training slides PDF, fluorophore guide, consumables list, quick reference guide Attune NxT Flow Cytometer support contact info For immediate assistance, please call: 1-80 (9:00 a.m.8:00 p.m.
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